| 质粒名称: | pcDNA3 Connexin43-GFP-APEX2 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 49385 |
| 存储实验室: | Alice Ting |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 空载体大小: | 5366 bp |
| 完整质粒大小: | 8042 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | XL1 Blue |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Connexin43 - EGFP - APEX2 |
| 物种来源: | soybean |
| 插入大小: | 2673 bp |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AscI(未破坏) |
| 3’克隆位点: | XhoI(未破坏) |
| 5’测序引物 | T7 |
| 3’测序引物 | BGHrev |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3 Connexin43-GFP-APEX2 was a gift from Alice Ting (Addgene plasmid # 49385 ; http://n2t.net/addgene:49385 ; RRID:Addgene_49385) |
| 参考文献部分: | Directed evolution of APEX2 for electron microscopy and proximity labeling. Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. 10.1038/nmeth.3179 PubMed 25419960 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA3 Connexin43-GFP-APEX2 8042 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43
in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pcDNA3 Connexin43-GFP-APEX2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8042)
AUTHORS Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK,
Ting AY
TITLE Directed evolution of APEX2 for electron microscopy and proximity
labeling.
JOURNAL Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
PUBMED 25419960
REFERENCE 2 (bases 1 to 8042)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8042
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 167..546
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 547..750
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 701..721
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 795..814
/label=T7
/note="T7 promoter, forward primer"
promoter 795..813
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 849..858
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2022..2738
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2067..2088)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2328..2347)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2675..2696
/label=EGFP-C
/note="EGFP, forward primer"
CDS 2778..3524
/codon_start=1
/product="soybean ascorbate peroxidase, engineered to serve
as a monomeric tag for electron microscopy and live-cell
proteomics (Lam et al., 2015)"
/label=APEX2
/note="more active than the original APEX due to the A134P
mutation"
/translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
YAADEDAFFADYAEAHQKLSELGFADA"
primer_bind complement(3565..3582)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3571..3795
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3841..4269
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3928..3947)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4138..4159
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4278..4298)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4283..4612
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4463..4598
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4525..4544
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4679..5473
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4733..4752)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5343..5362
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5647..5768
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5684..5703)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5738..5757
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5817..5833)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5817..5833)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5830..5852)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5841..5857
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5865..5895)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5910..5931
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6048..6065)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6219..6807)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6299..6318)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6978..7838)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7601..7620
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7839..7943)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8018..8037)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
61 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
121 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
181 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
241 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
301 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
361 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
421 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
481 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
541 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
601 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
661 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
721 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
781 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcgtttaaac
841 ttaagcttgc caccatgggt gactggagtg ccttggggaa attactggac aaggtccaag
901 cctactccac cgctggaggg aaggtgtggc tgtcagtgct cttcatattc agaatcctgc
961 tcctggggac agctgttgag tcagcttggg gtgatgaaca gtctgccttt cgctgtaaca
1021 ctcaacaacc tggctgcgaa aacgtctgct atgacaagtc cttccccatc tctcacgtgc
1081 gcttctgggt ccttcagatc atattcgtgt ctgtgcccac actcctgtac ttggcccatg
1141 tgttctatgt gatgaggaag gaagagaagc taaacaagaa agaagaggag ctcaaagtgg
1201 cccagactga cggggtcaac gtggagatgc acctgaagca gattgaaatc aagaagttca
1261 agtacgggat tgaagagcac ggcaaggtga aaatgagggg cggcttgctg agaacctaca
1321 tcatcagcat cctcttcaag tctgtcttcg aggtggcctt cctgctcatc cagtggtaca
1381 tctatgggtt cagcttgagc gcggtctaca cctgcaagag agatccctgc ccccaccagg
1441 tagactgctt cctctcacgt cccacggaga aaaccatctt catcatcttc atgctggtgg
1501 tgtccttggt gtctctcgct ttgaacatca ttgagctctt ctacgtcttc ttcaagggcg
1561 ttaaggatcg cgtgaaggga agaagcgatc cttaccacgc caccactggc ccactgagcc
1621 catcaaaaga ctgcggatct ccaaaatacg cctacttcaa tggctgctcc tcaccaacgg
1681 ctccactctc gcctatgtct cctcctgggt acaagctggt tactggtgac agaaacaatt
1741 cctcgtgccg caattacaac aagcaagcta gcgagcaaaa ctgggcgaac tacagcgcag
1801 agcaaaatcg catggggcag gccggaagca ccatctccaa ctcgcacgcc cagccgttcg
1861 atttccccga cgacaaccag aatgccaaaa aagttgctgc tggacatgaa ctccagccat
1921 tagccatcgt ggaccaacga ccttccagca gagccagcag ccgcgccagc agcaggcctc
1981 ggcctgatga cctggagatt ggatccgggg gtggtgggtc tatggtgagc aagggcgagg
2041 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
2101 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
2161 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
2221 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
2281 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
2341 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
2401 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
2461 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gccaacttca
2521 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
2581 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
2641 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
2701 ccgccgggat cactctcggc atggacgagc tgtacaaggg gcgcgccaag ggctcgggct
2761 cgacctcggg ctcgggcgga aagtcttacc caactgtgag tgctgattac caggacgccg
2821 ttgagaaggc gaagaagaag ctcagaggct tcatcgctga gaagagatgc gctcctctaa
2881 tgctccgttt ggcattccac tctgctggaa cctttgacaa gggcacgaag accggtggac
2941 ccttcggaac catcaagcac cctgccgaac tggctcacag cgctaacaac ggtcttgaca
3001 tcgctgttag gcttttggag ccactcaagg cggagttccc tattttgagc tacgccgatt
3061 tctaccagtt ggctggcgtt gttgccgttg aggtcacggg tggacctaag gttccattcc
3121 accctggaag agaggacaag cctgagccac caccagaggg tcgcttgccc gatcccacta
3181 agggttctga ccatttgaga gatgtgtttg gcaaagctat ggggcttact gaccaagata
3241 tcgttgctct atctgggggt cacactattg gagctgcaca caaggagcgt tctggatttg
3301 agggtccctg gacctctaat cctcttattt tcgacaactc atacttcacg gagttgttga
3361 gtggtgagaa ggaaggtctc cttcagctac cttctgacaa ggctcttttg tctgaccctg
3421 tattccgccc tctcgttgac aaatatgcag cggacgaaga tgccttcttt gctgattacg
3481 ctgaggctca ccaaaagctt tccgagcttg ggtttgctga tgcctaactc gagtctagag
3541 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
3601 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
3661 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
3721 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
3781 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
3841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
3901 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
3961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
4021 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
4081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
4141 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
4201 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
4261 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
4321 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
4381 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
4441 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
4501 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
4561 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
4621 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
4681 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
4741 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
4801 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
4861 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
4921 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
4981 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
5041 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
5101 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
5161 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
5221 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
5281 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
5341 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
5401 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
5461 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
5521 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
5581 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
5641 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
5701 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
5761 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
5821 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
5881 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
5941 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
6001 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
6061 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
6121 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
6181 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
6241 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
6301 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
6361 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
6421 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
6481 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
6541 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
6601 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
6661 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
6721 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
6781 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
6841 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
6901 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
6961 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
7021 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
7081 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
7141 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
7201 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
7261 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
7321 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
7381 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
7441 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
7501 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
7561 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
7621 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
7681 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
7741 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
7801 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
7861 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
7921 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
7981 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
8041 ag
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pNLS-iRFP713
- 下一篇：BC-GPR56
